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Overview of the Flexcell ® Tension system, mechanism of cell strain, and models used. (A) Schematic of the Flexcell ® FX-6000TM System, with components including: Surge protected power strip, power outlet, Ethernet cable, reinforced vacuum tubing, compressed air regulator/filter, vent tubing, system tubing, flex in tubing, flex out tubing, water trap, baseplate. To the right, enlargement of a top view of the baseplate in which the different <t>culture</t> <t>plates</t> sit for strain experiments showing white loading posts. (B) Schematic of top and side-view of membrane deformation and subsequent equibiaxial strain onto cells. (C) 2D alveolar epithelial-fibroblast co-cultures were seeded on BioFlex plates, whereas 3D co-cultures and epithelial-fibroblast organoids were seeded on the <t>Tissue</t> <t>Train</t> plate. Adapted from Flexcell ® International Corporation with written permission: USER MANUAL FLEXCELL ® DYNAMIC CULTURE SYSTEM FX-6000™ Tension 03-19-18 Rev 1.2, https://cdn.prod.website-files.com/5b916a469721fd4a8ae38199/5bb51aa931f6192010078b79_FX-6000TensionUsersManual.pdf , COPYRIGHT © 2018 FLEXCELL ® INTERNATIONAL CORPORATION, www.flexcellint.com ; FLEXCELL Loading Station Tech Report, Rev 7.0 07-25-17, https://cdn.prod.website-files.com/5b916a469721fd4a8ae38199/5bb519b8e56d4d5aedf7e3c8_101_LoadingStationsTech.pdf , COPYRIGHT © 2009 FLEXCELL ® INTERNATIONAL CORPORATION, www.flexcellint.com .
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Overview of the Flexcell ® Tension system, mechanism of cell strain, and models used. (A) Schematic of the Flexcell ® FX-6000TM System, with components including: Surge protected power strip, power outlet, Ethernet cable, reinforced vacuum tubing, compressed air regulator/filter, vent tubing, system tubing, flex in tubing, flex out tubing, water trap, baseplate. To the right, enlargement of a top view of the baseplate in which the different <t>culture</t> <t>plates</t> sit for strain experiments showing white loading posts. (B) Schematic of top and side-view of membrane deformation and subsequent equibiaxial strain onto cells. (C) 2D alveolar epithelial-fibroblast co-cultures were seeded on BioFlex plates, whereas 3D co-cultures and epithelial-fibroblast organoids were seeded on the <t>Tissue</t> <t>Train</t> plate. Adapted from Flexcell ® International Corporation with written permission: USER MANUAL FLEXCELL ® DYNAMIC CULTURE SYSTEM FX-6000™ Tension 03-19-18 Rev 1.2, https://cdn.prod.website-files.com/5b916a469721fd4a8ae38199/5bb51aa931f6192010078b79_FX-6000TensionUsersManual.pdf , COPYRIGHT © 2018 FLEXCELL ® INTERNATIONAL CORPORATION, www.flexcellint.com ; FLEXCELL Loading Station Tech Report, Rev 7.0 07-25-17, https://cdn.prod.website-files.com/5b916a469721fd4a8ae38199/5bb519b8e56d4d5aedf7e3c8_101_LoadingStationsTech.pdf , COPYRIGHT © 2009 FLEXCELL ® INTERNATIONAL CORPORATION, www.flexcellint.com .
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Overview of the Flexcell ® Tension system, mechanism of cell strain, and models used. (A) Schematic of the Flexcell ® FX-6000TM System, with components including: Surge protected power strip, power outlet, Ethernet cable, reinforced vacuum tubing, compressed air regulator/filter, vent tubing, system tubing, flex in tubing, flex out tubing, water trap, baseplate. To the right, enlargement of a top view of the baseplate in which the different culture plates sit for strain experiments showing white loading posts. (B) Schematic of top and side-view of membrane deformation and subsequent equibiaxial strain onto cells. (C) 2D alveolar epithelial-fibroblast co-cultures were seeded on BioFlex plates, whereas 3D co-cultures and epithelial-fibroblast organoids were seeded on the Tissue Train plate. Adapted from Flexcell ® International Corporation with written permission: USER MANUAL FLEXCELL ® DYNAMIC CULTURE SYSTEM FX-6000™ Tension 03-19-18 Rev 1.2, https://cdn.prod.website-files.com/5b916a469721fd4a8ae38199/5bb51aa931f6192010078b79_FX-6000TensionUsersManual.pdf , COPYRIGHT © 2018 FLEXCELL ® INTERNATIONAL CORPORATION, www.flexcellint.com ; FLEXCELL Loading Station Tech Report, Rev 7.0 07-25-17, https://cdn.prod.website-files.com/5b916a469721fd4a8ae38199/5bb519b8e56d4d5aedf7e3c8_101_LoadingStationsTech.pdf , COPYRIGHT © 2009 FLEXCELL ® INTERNATIONAL CORPORATION, www.flexcellint.com .

Journal: Frontiers in Medicine

Article Title: Dynamic mechanical stimulation of alveolar epithelial-fibroblast models using the Flexcell tension system to study of lung disease mechanisms

doi: 10.3389/fmed.2025.1552803

Figure Lengend Snippet: Overview of the Flexcell ® Tension system, mechanism of cell strain, and models used. (A) Schematic of the Flexcell ® FX-6000TM System, with components including: Surge protected power strip, power outlet, Ethernet cable, reinforced vacuum tubing, compressed air regulator/filter, vent tubing, system tubing, flex in tubing, flex out tubing, water trap, baseplate. To the right, enlargement of a top view of the baseplate in which the different culture plates sit for strain experiments showing white loading posts. (B) Schematic of top and side-view of membrane deformation and subsequent equibiaxial strain onto cells. (C) 2D alveolar epithelial-fibroblast co-cultures were seeded on BioFlex plates, whereas 3D co-cultures and epithelial-fibroblast organoids were seeded on the Tissue Train plate. Adapted from Flexcell ® International Corporation with written permission: USER MANUAL FLEXCELL ® DYNAMIC CULTURE SYSTEM FX-6000™ Tension 03-19-18 Rev 1.2, https://cdn.prod.website-files.com/5b916a469721fd4a8ae38199/5bb51aa931f6192010078b79_FX-6000TensionUsersManual.pdf , COPYRIGHT © 2018 FLEXCELL ® INTERNATIONAL CORPORATION, www.flexcellint.com ; FLEXCELL Loading Station Tech Report, Rev 7.0 07-25-17, https://cdn.prod.website-files.com/5b916a469721fd4a8ae38199/5bb519b8e56d4d5aedf7e3c8_101_LoadingStationsTech.pdf , COPYRIGHT © 2009 FLEXCELL ® INTERNATIONAL CORPORATION, www.flexcellint.com .

Article Snippet: 3D co-culture models were created in the 6 well Tissue Train circular foam culture plates (Flexcell International Corporation) by resuspending 100,000 MRC-5 lung fibroblasts in 2 mg/mL rat tail collagen I per well as per the manufacturer's protocol (Thermo Fisher Scientific, A10483-01).

Techniques: Stripping Membranes, Membrane